Protein-protein interactions and nuclear trafficking of coat protein and βC1 protein associated with Bhendi yellow vein mosaic disease

P. Kumar P.; R. Usha; A. Zrachya; Y. Levy; H. Spanov; Y. Gafni

doi:10.1016/j.virusres.2006.07.007

Protein-protein interactions and nuclear trafficking of coat protein and βC1 protein associated with Bhendi yellow vein mosaic disease

P. Kumar P., R. Usha, A. Zrachya, Y. Levy, H. Spanov, Y. Gafni

Research output: Contribution to journal › Article › peer-review

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Abstract

Bhendi yellow vein mosaic disease (BYVMD) is caused by a complex consisting of a monopartite begomovirus BYVMV and a satellite DNA β component. BYVMV represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction. Here we report the results of the transient expression of green fluorescent protein (GFP) fused with the βC1 and coat protein (CP) coding regions, in the epidermal cells of Nicotiana benthamiana. GFPCP was found to be targeted into the nucleus whereas GFPβC1 was localized towards the periphery of the cell. The sub-cellular localization of the βC1 protein has been compared with that of the CP in yeast cells using a genetic system for detection of protein nuclear import and export. Expression of βC1 ORF in transgenic N. benthamiana under the control of the Cauliflower mosaic virus 35S promoter produced severe developmental abnormalities in the plant, like distorted stem, leaves and stunting of the plant. We also present the results on the interaction of CP and βC1 proteins using yeast two hybrid analysis, suggesting a collaborative role in the inter- and intracellular dynamics of BYVMD.

Original language	English
Pages (from-to)	127-136
Number of pages	10
Journal	Virus Research
Volume	122
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2006.07.007
State	Published - Dec 2006
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by funding from CSIR, UGC (Centre for potential in the subject of genomic sciences), DST and DBT of India and the MASHAV of Israel. The instrument facilities provided by DST-FIST and DBT are acknowledged. Senior research fellowship (CSIR) and MASHAV fellowship to P.P.K. are gratefully acknowledged. We thank Dr. V. Citovsky for the yeast one-hybrid vectors. We thank Mr. Eddy Blasov for technical assistance in confocal microscopy. We thank Prof. K. Veluthambi for pGA643 and LBA4404. We are grateful to Ms. K. Vydehi, Mr. P. Gopal and Dr. B. Sinilal for helpful discussions.

Funding

This work was supported by funding from CSIR, UGC (Centre for potential in the subject of genomic sciences), DST and DBT of India and the MASHAV of Israel. The instrument facilities provided by DST-FIST and DBT are acknowledged. Senior research fellowship (CSIR) and MASHAV fellowship to P.P.K. are gratefully acknowledged. We thank Dr. V. Citovsky for the yeast one-hybrid vectors. We thank Mr. Eddy Blasov for technical assistance in confocal microscopy. We thank Prof. K. Veluthambi for pGA643 and LBA4404. We are grateful to Ms. K. Vydehi, Mr. P. Gopal and Dr. B. Sinilal for helpful discussions.

Funders	Funder number
Council for Scientific and Industrial Research, South Africa
Department of Science and Technology, Republic of South Africa
Department of Biotechnology, Ministry of Science and Technology, India
University Grants Committee

Keywords

Begomovirus
Bhendi yellow vein mosaic virus
Coat protein
Nuclear export
Nuclear localization
βC1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2006.07.007

Cite this

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title = "Protein-protein interactions and nuclear trafficking of coat protein and βC1 protein associated with Bhendi yellow vein mosaic disease",

abstract = "Bhendi yellow vein mosaic disease (BYVMD) is caused by a complex consisting of a monopartite begomovirus BYVMV and a satellite DNA β component. BYVMV represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction. Here we report the results of the transient expression of green fluorescent protein (GFP) fused with the βC1 and coat protein (CP) coding regions, in the epidermal cells of Nicotiana benthamiana. GFPCP was found to be targeted into the nucleus whereas GFPβC1 was localized towards the periphery of the cell. The sub-cellular localization of the βC1 protein has been compared with that of the CP in yeast cells using a genetic system for detection of protein nuclear import and export. Expression of βC1 ORF in transgenic N. benthamiana under the control of the Cauliflower mosaic virus 35S promoter produced severe developmental abnormalities in the plant, like distorted stem, leaves and stunting of the plant. We also present the results on the interaction of CP and βC1 proteins using yeast two hybrid analysis, suggesting a collaborative role in the inter- and intracellular dynamics of BYVMD.",

keywords = "Begomovirus, Bhendi yellow vein mosaic virus, Coat protein, Nuclear export, Nuclear localization, βC1",

author = "{Kumar P.}, P. and R. Usha and A. Zrachya and Y. Levy and H. Spanov and Y. Gafni",

note = "Funding Information: This work was supported by funding from CSIR, UGC (Centre for potential in the subject of genomic sciences), DST and DBT of India and the MASHAV of Israel. The instrument facilities provided by DST-FIST and DBT are acknowledged. Senior research fellowship (CSIR) and MASHAV fellowship to P.P.K. are gratefully acknowledged. We thank Dr. V. Citovsky for the yeast one-hybrid vectors. We thank Mr. Eddy Blasov for technical assistance in confocal microscopy. We thank Prof. K. Veluthambi for pGA643 and LBA4404. We are grateful to Ms. K. Vydehi, Mr. P. Gopal and Dr. B. Sinilal for helpful discussions.",

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AU - Levy, Y.

AU - Spanov, H.

AU - Gafni, Y.

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Protein-protein interactions and nuclear trafficking of coat protein and βC1 protein associated with Bhendi yellow vein mosaic disease

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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