Bhendi yellow vein mosaic disease (BYVMD) is caused by a complex consisting of a monopartite begomovirus BYVMV and a satellite DNA β component. BYVMV represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction. Here we report the results of the transient expression of green fluorescent protein (GFP) fused with the βC1 and coat protein (CP) coding regions, in the epidermal cells of Nicotiana benthamiana. GFPCP was found to be targeted into the nucleus whereas GFPβC1 was localized towards the periphery of the cell. The sub-cellular localization of the βC1 protein has been compared with that of the CP in yeast cells using a genetic system for detection of protein nuclear import and export. Expression of βC1 ORF in transgenic N. benthamiana under the control of the Cauliflower mosaic virus 35S promoter produced severe developmental abnormalities in the plant, like distorted stem, leaves and stunting of the plant. We also present the results on the interaction of CP and βC1 proteins using yeast two hybrid analysis, suggesting a collaborative role in the inter- and intracellular dynamics of BYVMD.
|Number of pages||10|
|State||Published - Dec 2006|
Bibliographical noteFunding Information:
This work was supported by funding from CSIR, UGC (Centre for potential in the subject of genomic sciences), DST and DBT of India and the MASHAV of Israel. The instrument facilities provided by DST-FIST and DBT are acknowledged. Senior research fellowship (CSIR) and MASHAV fellowship to P.P.K. are gratefully acknowledged. We thank Dr. V. Citovsky for the yeast one-hybrid vectors. We thank Mr. Eddy Blasov for technical assistance in confocal microscopy. We thank Prof. K. Veluthambi for pGA643 and LBA4404. We are grateful to Ms. K. Vydehi, Mr. P. Gopal and Dr. B. Sinilal for helpful discussions.
- Bhendi yellow vein mosaic virus
- Coat protein
- Nuclear export
- Nuclear localization