Protein kinase inhibitors induce the interphase transition by inactivating mitogen-activated protein kinase in mouse eggs

Qing Yuan Sun, Ayala Luria, Sara Rubinstein, Haim Breitbart

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    The role of mitogen-activated protein (MAP) kinase in mouse egg activation induced by protein kinase inhibitors and a protein tyrosine kinase (PTK) inhibitor was investigated. Separated egg proteins were first probed with anti-Active MAP kinase antibody and then re-probed with anti-ERK2 antibody. Staurosporine and Ro-31-8220, at concentrations that normally inhibit protein kinase C, did not affect egg activation or MAP kinase activity, while higher dosages caused egg activation. Staurosporine at 2 μM induced the metaphase-interphase transition without emission of the second polar body (PB2), while Ro-31-8220 at 40 μM induced PB2 emission, first cleavage, and then the transition to interphase. Half the eggs were also activated by the PTK inhibitor genistein. In each treatment, the proportion of eggs that entered interphase was well correlated with the degree of MAP kinase inactivation. Artificial activation of this kinase by okadaic acid overcame the interphase transition. These data suggest that protein kinase inhibitors and a protein tyrosine kinase inhibitor induce the interphase transition by inactivating MAP kinase in mouse eggs.

    Original languageEnglish
    Pages (from-to)277-284
    Number of pages8
    JournalZygote
    Volume6
    Issue number3
    DOIs
    StatePublished - Aug 1998

    Keywords

    • Egg activation
    • MAP kinase
    • Mouse
    • Protein kinase inhibitor
    • Protein tyrosine kinase

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