Protein kinase C (PKC) isoenzymes immunohistochemistry in lymph node revealing solution-fixed, paraffin-embedded bladder tumors

Protein kinase C (PKC) plays an important role in cellular differentiation and in the malignant process. In an earlier study, it was shown that the expression pattern of PKC isoenzymes is altered in some tumors compared to their corresponding normal tissue. In this study, we evaluated the pattern of PKC isoenzyme immunostaining in bladder transitional cell carcinoma (TCC) of different grades and stages and normal tissue. Twenty- seven TCC samples and six areas of normal bladder mucosa were stained with antibodies specific for the PKC isoenzymes: α, β1, β2, δ, and ζ. The sections were scored for intensity of staining, and the correlation with grade and stage of the tumors was computed. The PKC α and β2 immunostains were intense in normal urothelium and in all evaluated tumors. PKC β1 and δ stains were intense in normal and low-grade and -stage tumors and weak in high-grade and -stage tumors. The opposite trend was found for PKC ζ. PKC isoenzyme expression differs in invasive TCC compared to low-grade, low-stage TCC and normal urothelium. The value of these findings as a marker of tumor aggressiveness should be further assessed.