Proteasome storage granules are transiently associated with the insoluble protein deposit in Saccharomyces cerevisiae

Lee Zeev Peters, Ofri Karmon, Shir Miodownik, Shay Ben-Aroya

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Proteasome storage granules (PSGs) are created in yeast as part of an extensive and programmed reorganization of proteins into reversible assemblies upon carbon source depletion. Here, we demonstrate that cells distinguish dysfunctional proteasomes from PSGs on the cytosolic insoluble protein deposit (IPOD). Furthermore, we provide evidence that this is a general mechanism for the reorganization of additional proteins into reversible assemblies. Our study expands the roles of the IPOD, which might serve not only as the specific depository for amyloidogenic and misfolded proteins, but also as a potential hub from which proteins are directed to distinct cellular compartments. These findings therefore provide a framework for understanding how cells discriminate between intact and abnormal proteins under stress conditions to ensure that only structurally 'correct' proteins are deployed.

Original languageEnglish
Pages (from-to)1190-1197
Number of pages8
JournalJournal of Cell Science
Volume129
Issue number6
DOIs
StatePublished - 15 Mar 2016

Bibliographical note

Publisher Copyright:
© 2016. Published by The Company of Biologists Ltd.

Keywords

  • IPOD
  • Insoluble protein deposit
  • PSGs
  • Proteasome
  • Proteasome storage granules
  • Protein quality control
  • Saccharomyces cerevisiae

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