Promotion of neural sprouting using low-level green light-emitting diode phototherapy

Noa Alon; Hamootal Duadi; Ortal Cohen; Tamar Samet; Neta Zilony; Hadas Schori; Orit Shefi; Zeev Zalevsky

doi:10.1117/1.JBO.20.2.020502

Promotion of neural sprouting using low-level green light-emitting diode phototherapy

Noa Alon, Hamootal Duadi, Ortal Cohen, Tamar Samet, Neta Zilony, Hadas Schori, Orit Shefi, Zeev Zalevsky

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm². We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.

Original language	English
Article number	020502
Journal	Journal of Biomedical Optics
Volume	20
Issue number	2
DOIs	https://doi.org/10.1117/1.JBO.20.2.020502
State	Published - 1 Feb 2015

Bibliographical note

Publisher Copyright:
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).

Keywords

light-emitting diode illumination
neural sprouting
phototherapy

Access to Document

10.1117/1.JBO.20.2.020502

Cite this

@article{acc5f7d7421042479e6015f427792afb,

title = "Promotion of neural sprouting using low-level green light-emitting diode phototherapy",

abstract = "We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.",

keywords = "light-emitting diode illumination, neural sprouting, phototherapy",

author = "Noa Alon and Hamootal Duadi and Ortal Cohen and Tamar Samet and Neta Zilony and Hadas Schori and Orit Shefi and Zeev Zalevsky",

note = "Publisher Copyright: {\textcopyright} 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).",

year = "2015",

month = feb,

day = "1",

doi = "10.1117/1.JBO.20.2.020502",

language = "אנגלית",

volume = "20",

journal = "Journal of Biomedical Optics",

issn = "1083-3668",

publisher = "SPIE",

number = "2",

}

TY - JOUR

T1 - Promotion of neural sprouting using low-level green light-emitting diode phototherapy

AU - Alon, Noa

AU - Duadi, Hamootal

AU - Cohen, Ortal

AU - Samet, Tamar

AU - Zilony, Neta

AU - Schori, Hadas

AU - Shefi, Orit

AU - Zalevsky, Zeev

PY - 2015/2/1

Y1 - 2015/2/1

N2 - We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.

AB - We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.

KW - light-emitting diode illumination

KW - neural sprouting

KW - phototherapy

UR - http://www.scopus.com/inward/record.url?scp=84922569791&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.20.2.020502

DO - 10.1117/1.JBO.20.2.020502

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 25652701

AN - SCOPUS:84922569791

SN - 1083-3668

VL - 20

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 2

M1 - 020502

ER -

Promotion of neural sprouting using low-level green light-emitting diode phototherapy

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this