TY - JOUR
T1 - Promotion of neural sprouting using low-level green light-emitting diode phototherapy
AU - Alon, Noa
AU - Duadi, Hamootal
AU - Cohen, Ortal
AU - Samet, Tamar
AU - Zilony, Neta
AU - Schori, Hadas
AU - Shefi, Orit
AU - Zalevsky, Zeev
N1 - Publisher Copyright:
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2015/2/1
Y1 - 2015/2/1
N2 - We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.
AB - We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100 mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.
KW - light-emitting diode illumination
KW - neural sprouting
KW - phototherapy
UR - http://www.scopus.com/inward/record.url?scp=84922569791&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.20.2.020502
DO - 10.1117/1.JBO.20.2.020502
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C2 - 25652701
AN - SCOPUS:84922569791
SN - 1083-3668
VL - 20
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 2
M1 - 020502
ER -