Prolonged controlled delivery of nerve growth factor using porous silicon nanostructures

Neta Zilony, Michal Rosenberg, Liran Holtzman, Hadas Schori, Orit Shefi, Ester Segal

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Although nerve growth factor (NGF) is beneficial for the treatment of numerous neurological and non-neurological diseases, its therapeutic administration represents a significant challenge, due to the difficulty to locally deliver relevant doses in a safe and non-invasive manner. In this work, we employ degradable nanostructured porous silicon (PSi) films as carriers for NGF, allowing its continuous and prolonged release, while retaining its bioactivity. The PSi carriers exhibit high loading efficacy (up to 90%) of NGF and a continuous release, with no burst, over a period of > 26 days. The released NGF bioactivity is compared to that of free NGF in both PC12 cells and dissociated dorsal root ganglion (DRG) neurons. We show that the NGF has retained its bioactivity and induces neurite outgrowth and profound differentiation (of > 50% for PC12 cells) throughout the period of release within a single administration. Thus, this proof-of-concept study demonstrates the immense therapeutic potential of these tunable carriers as long-term implants of NGF reservoirs and paves the way for new localized treatment strategies of neurodegenerative diseases.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalJournal of Controlled Release
Volume257
DOIs
StatePublished - 10 Jul 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

  • Controlled release
  • Differentiation
  • Dorsal root ganglia
  • Nerve growth factor
  • PC12
  • Porous silicon

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