Profile of gene expression regulated by induced p53: Connection to the TGF-β family

Karuppiah Kannan; Ninette Amariglio; Gideon Rechavi; David Givol

doi:10.1016/S0014-5793(00)01291-6

Profile of gene expression regulated by induced p53: Connection to the TGF-β family

Karuppiah Kannan, Ninette Amariglio, Gideon Rechavi, David Givol

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The transcription regulatory function of p53 was analyzed by using two inducible p53 systems in the human lung cancer cell line H1299. cDNA probes derived from RNA harvested 12 h after p53 induction were used to probe filters containing cDNA arrays. Over 20 genes were found to be significantly induced or suppressed by p53. The induced genes can be classified mainly as cell cycle inhibitors like p21waf, GADD45, apoptosis-related genes like Fas/APO1 and PIG3 or DNA repair genes like DDB2, DNA ligase and G/T mismatch DNA glycosylase. The suppressed genes include mainly cell cycle regulators like cyclin B1, cyclin H and kinases like c-abl, CLK1 and others. The most notable induced gene was MIC-1, encoding a TGF-β-related secretory protein, suggesting a potential paracrine component for p53 growth suppression. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	77-82
Number of pages	6
Journal	FEBS Letters
Volume	470
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)01291-6
State	Published - 17 Mar 2000
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported in part by Yad Abraham Research Center for Cancer Diagnosis and Therapy and the Rich Foundation for Leukemia Research. G.R. holds the Gregorio and Dora Shapiro Chair for Hematologic Malignancies, Sackler School of Medicine, Tel Aviv University. We thank Drs. A. Hochberg and A. Suhail from The Hebrew University, Jerusalem, Israel, for helpful discussions concerning array image analysis and Dr. M. Oren for p53Val135 expressing H1299 cells.

Funding

This work was supported in part by Yad Abraham Research Center for Cancer Diagnosis and Therapy and the Rich Foundation for Leukemia Research. G.R. holds the Gregorio and Dora Shapiro Chair for Hematologic Malignancies, Sackler School of Medicine, Tel Aviv University. We thank Drs. A. Hochberg and A. Suhail from The Hebrew University, Jerusalem, Israel, for helpful discussions concerning array image analysis and Dr. M. Oren for p53Val135 expressing H1299 cells.

Funders	Funder number
Rich Foundation for Leukemia Research
Yad Abraham Research Center for Cancer Diagnosis and Therapy

Keywords

DNA array
DNA repair
MIC-1
Transactivation
Transrepression
p53

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0014-5793(00)01291-6

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abstract = "The transcription regulatory function of p53 was analyzed by using two inducible p53 systems in the human lung cancer cell line H1299. cDNA probes derived from RNA harvested 12 h after p53 induction were used to probe filters containing cDNA arrays. Over 20 genes were found to be significantly induced or suppressed by p53. The induced genes can be classified mainly as cell cycle inhibitors like p21waf, GADD45, apoptosis-related genes like Fas/APO1 and PIG3 or DNA repair genes like DDB2, DNA ligase and G/T mismatch DNA glycosylase. The suppressed genes include mainly cell cycle regulators like cyclin B1, cyclin H and kinases like c-abl, CLK1 and others. The most notable induced gene was MIC-1, encoding a TGF-β-related secretory protein, suggesting a potential paracrine component for p53 growth suppression. (C) 2000 Federation of European Biochemical Societies.",

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Profile of gene expression regulated by induced p53: Connection to the TGF-β family

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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