Production of Human Lymphoblastoid (Namalva) Interferon

The cell substrates that are used for human lymphoblastoid interferon (HLyIF) production are derived from human lymphoblastoid cell lines, most of which are established from Burkitt's lymphoma cases. Such cells are grown in submerged culture, and after suitable viral induction, some lines produced HLyIF in reasonable amounts. Human lymphoblastoid cells are first screened for interferon production. Large amounts are produced by several lymphoblastoid cell lines, the best of which is found to be the Namalva lymphoblastoid cell line. Most production of HLyIF is now being carried out with the Namalva cells. For separation of cells, the fequipment that may be used are batch and continuous-flow centrifuges, bottle laboratory centrifuge, industrialscale tubular continuous-flow centrifuge, and basket centrifuges. There are three main steps in the production of HLyIF: Cell propagation, HLyIF induction, and HLyIF purification. Namalva cells are then available for IF induction. Using this method, several parallel lines of scaled-up cultures should be maintained continuously.