Production of carcinoembryonic antigen from a human colon adenocarcinoma cell line. I. Large-scale cultivation of carcinoembryonic antigen-producing cells on cylindric cellulose-based microcarriers.

A. Lazar, S. Reuveny, J. Geva, D. Marcus, L. Silberstein, N. Ariel, N. Epstein, Z. Altbaum, J. Sinai, A. Mizrahi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A continuous cell line from a human colon carcinoma, designated HuCC1-14, was successfully grown on cyclindrical cellulose microcarriers (MC) charged by DEAE. The cells grow on these carriers as large cell-MC aggregates and maintain high cell densities in submerged conditions for extended periods. HuCC1-14 cells secrete into the culture medium significant amounts of carcinoembryonic antigen (CEA). Maximum efficiency in CEA secretion occurred after the cells switched from logarithmic growth to stationary phase. At this state, high CEA levels could be obtained with low-serum medium which greatly facilitates subsequent product purification. The described method provides a system which can be scaled-up and produce this tumor-associated antigen in essentially unlimited amounts and reproducible quality for detection and monitoring of cancer patients.

Original languageEnglish
Pages (from-to)423-428
Number of pages6
JournalDevelopments in Biological Standardization
Volume66
StatePublished - 1987

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