Production and Characterization of Egg-Derived Antibodies (IgY) Against Outer Membrane Proteins of Multidrug-Resistant Salmonella enterica Serovar Typhimurium

Non-typhoidal salmonellosis is a globally prevalent zoonotic disease, often associated with the growing concern of antimicrobial resistance (AMR). This persistent issue underscores the urgent need for novel strategies for prevention and control. Although conventional approaches such as drug or vaccine development are widely explored, they are often time-consuming, costly, and subject to potential bacterial resistance over time. As an alternative, egg-derived antibodies (IgY) offer a promising solution. IgY can be produced in large quantities without the need for complex infrastructure or environmental risk. Their production is characterized by a favorable safety profile, scalability, high antibody yield per egg yolk, cost-effectiveness, and lack of cross-reactivity in mammals—attributes that collectively make this technology highly advantageous. The present investigation aimed to establish a standardized protocol for hyperimmunization, production, and isolation of IgY antibodies from the yolk of immunized hens targeting multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium. The study further focused on the characterization and specificity assessment of the purified IgY. The findings suggest that the resulting IgY holds strong potential as a therapeutic, prophylactic, or diagnostic agent against MDR S. Typhimurium.