Problems and approaches in covalent attachment of peptides and proteins to inorganic surfaces for biosensor applications

James E. Zull, J. Reed-Mundell, Y. W. Lee, Dmitri Vezenov, Nicholas P. Ziats, James M. Anderson, Chaim N. Sukenik

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations

Abstract

Some of the fundamental problems in covalent attachment of peptides and proteins to putative biosensor surfaces are reviewed and specific approaches to these problems discussed. In addition, selected aspects of our recent work utilizing self-assembled monolayer (SAM) systems designed to react selectively with the thiol side chain of Cys in proteins are presented. Uniform attachment of a 21-amino acid peptide antigen through a single Cys residue with retention of biological function (antibody binding) has been attained. Further work with this system may lead to solutions for some of the problems which currently prevent the development of reliable biosensors for industrial and medical use.

Original languageEnglish
Pages (from-to)137-143
Number of pages7
JournalJournal of Industrial Microbiology
Volume13
Issue number3
DOIs
StatePublished - May 1994
Externally publishedYes

Funding

FundersFunder number
National Heart, Lung, and Blood InstituteR01HL033849

    Keywords

    • Biosensors
    • Monolayer films

    Fingerprint

    Dive into the research topics of 'Problems and approaches in covalent attachment of peptides and proteins to inorganic surfaces for biosensor applications'. Together they form a unique fingerprint.

    Cite this