A novel method for preparation of durable standards of biological samples for quantitative analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed. Rat brain tissue samples were ground and then spiked by aqueous solutions containing controlled amounts of trace metals of interest. The spiked rat brain samples were then encapsulated in a sol-gel matrix produced by tetraethyl orthosilicate (TEOS). The spiked standards were prepared by addition of 0-50 μg g-1 of eight elements (Li, Co, Cr, Cu, Fe, Mn, Pb and Zn) to the biological samples. The correlation coefficients of the calibration curves for all elements tested were better than 0.97. Carbon-13, found in all biological samples, was used as an internal standard to correct for variations in the amount of material ablated and carried to the ICP-MS plasma torch, a common problem in LA-ICP-MS analysis. The results of analysis of the standards showed very good repeatability and homogeneity as well as long room temperature shelf life.
|Number of pages||7|
|Journal||International Journal of Mass Spectrometry|
|State||Published - 1 Oct 2011|
Bibliographical noteFunding Information:
The authors would like to acknowledge the support from the Geological Survey of Israel (GSI), Jerusalem for letting us use their facilities. Special thank given to Dr. Ludwik Halicz for his help and support throughout the project.
- Biological samples
- Laser ablation ICP-MS
- Sol-gel matrix
- Trace elements