Preparation of multivesicular liposomes

Sinil Kim; Mitchell S. Turker; Emil Y. Chi; Shifra Sela; George M. Martin

doi:10.1016/0005-2736(83)90504-7

Preparation of multivesicular liposomes

Sinil Kim, Mitchell S. Turker, Emil Y. Chi, Shifra Sela, George M. Martin

University of Washington

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

Original language	English
Pages (from-to)	339-348
Number of pages	10
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	728
Issue number	3
DOIs	https://doi.org/10.1016/0005-2736(83)90504-7
State	Published - 9 Mar 1983
Externally published	Yes

Bibliographical note

Funding Information:
We thank Mr. Chuan Teh, Mr. Daniel Teh and Ms. Marguerite Knutson for technical assistance. We also thank Dr. Dave A. Kalman of the Department of Environmental Health, University of Washington, for gas chromatography. This work was supported by Grants AG 01751, AG 00057 and GM 15253 from the NIH.

Funding

We thank Mr. Chuan Teh, Mr. Daniel Teh and Ms. Marguerite Knutson for technical assistance. We also thank Dr. Dave A. Kalman of the Department of Environmental Health, University of Washington, for gas chromatography. This work was supported by Grants AG 01751, AG 00057 and GM 15253 from the NIH.

Funders	Funder number
National Institutes of Health
National Institute on Aging	P01AG001751
National Institute of General Medical Sciences	P50GM015253

Keywords

Encapsulation
Liposome preparation

Access to Document

10.1016/0005-2736(83)90504-7

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title = "Preparation of multivesicular liposomes",

abstract = "A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.",

keywords = "Encapsulation, Liposome preparation",

author = "Sinil Kim and Turker, {Mitchell S.} and Chi, {Emil Y.} and Shifra Sela and Martin, {George M.}",

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N2 - A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

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Preparation of multivesicular liposomes

Abstract

Bibliographical note

Funding

Keywords

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