Prediction of antigenic binders from c-terminal domain human papillomavirus oncoprotein e7

Virendra S. Gomase, Somnath Tagore, Krishnan Shyamkumar

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Human papillomavirus (HPV) is one of the most common causes of sexually transmitted disease (STD). Human papillomavirus viral peptides are most suitable for subunit vaccine development because with single epitope, the immune response can be generated in large population. TAP is a transporter associated with MHC class I restricted antigen processing. The TAP is heterodimeric transporter belong to the family of ABC transporter, that uses the energy provided by ATP to translocate the peptides across the membrane. The subset of this transported peptide will bind MHC class II molecules and stabilize them. These MHC-peptide complexes will be translocated on the surface of antigen presenting cells (APCs). In this assay we predicted the binding affinity of Human papillomavirus oncoprotein e7 having 56 amino acids, which shows 49 nonamers. Small peptide regions found as 9-RHKILCVCC (score 6.186), 34-LRTLQQLFL (Score- 6.091), 31-AEDLRTLQQ (Score- 5.979), 8-QRHKILCVC (Score- 5.960), 45-LSFVCPWCA (Score-5.604), known as oncoprotein e7 TAP transporter. Adducts of MHC and peptide complexes are the ligands for T cell receptors (TCR). These complexes elicit the immune response for clearing various intracellular infections. Prediction methods based on the specificity of TAP transporter will complement the wet lab experiments and speed up the knowledge discoveries on the basis of these two computational algorithms.

Original languageEnglish
Pages (from-to)147-166
Number of pages20
JournalGene Therapy and Molecular Biology
Volume12
Issue number2
StatePublished - 2008
Externally publishedYes

Keywords

  • APCs
  • MHC
  • Oncoprotein e7
  • TAP transporter
  • TCR

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