Preclinical evidence of mitochondrial nicotinamide adenine dinucleotide as an effective alarm parameter under hypoxia

Hua Shi, Nannan Sun, Avraham Mayevsky, Zhihong Zhang, Qingming Luo

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11 Scopus citations

Abstract

Early detection of tissue hypoxia in the intensive care unit is essential for effective treatment. Reduced nicotinamide adenine dinucleotide (NADH) has been suggested to be the most sensitive indicator of tissue oxygenation at the mitochondrial level. However, no experimental evidence comparing the kinetics of changes in NADH and other physiological parameters has been provided. The aim of this study is to obtain the missing data in a systematic and reliable manner. We constructed four acute hypoxia models, including hypoxic hypoxia, hypemic hypoxia, circulatory hypoxia, and histogenous hypoxia, and measured NADH fluorescence, tissue reflectance, cerebral blood flow, respiration, and electrocardiography simultaneously from the induction of hypoxia until death. We found that NADH was not always the first onset parameter responding to hypoxia. The order of responses was mainly affected by the cause of hypoxia. However, NADH reached its alarm level earlier than the other monitored parameters, ranging from several seconds to >10 min. As such, we suggest that the NADH can be used as a hypoxia indicator, although the exact level that should be used must be further investigated. When the NADH alarm is detected, the body still has a chance to recover if appropriate and timely treatment is provided.

Original languageEnglish
Article number017005
JournalJournal of Biomedical Optics
Volume19
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

Bibliographical note

Funding Information:
Avraham Mayevsky is on leave from The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 52900 Ramat Gan, Israel, and is supported by the 111 Project of China (B07038). This work is also supported by the PhD Programs Foundation of the Ministry of Education of China (Grant No. 20110142130006) and the Director Fund of Wuhan National Laboratory for Optoelectronics (WNLO, 2009, Z. H. Zhang).

Funding

Avraham Mayevsky is on leave from The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 52900 Ramat Gan, Israel, and is supported by the 111 Project of China (B07038). This work is also supported by the PhD Programs Foundation of the Ministry of Education of China (Grant No. 20110142130006) and the Director Fund of Wuhan National Laboratory for Optoelectronics (WNLO, 2009, Z. H. Zhang).

FundersFunder number
Mina & Everard Goodman Faculty of Life Sciences
Ministry of Education of the People's Republic of China20110142130006
Bar-Ilan University52900
Higher Education Discipline Innovation ProjectB07038

    Keywords

    • Alarm
    • Hypoxia
    • Intensive care
    • Monitoring
    • Nicotinamide adenine dinucleotide fluorescence
    • Oxygen indicator

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