TY - JOUR
T1 - Porous gelatin hydrogels
T2 - 2. In vitro cell interaction study
AU - Dubruel, Peter
AU - Unger, R.
AU - Van Vlierberghe, Sandra
AU - Cnudde, Veerle
AU - Jacobs, Patric J.S.
AU - Schacht, Etienne
AU - Kirkpatrick, C. J.
PY - 2007/2
Y1 - 2007/2
N2 - We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330,μm) to the bottom (20-30 μm). Type II hydrogels contained spherical pores with a diameter of 135,μm. Both types of scaffolds were evaluated by confocal laser scanning microscopy in terms of adhesion, spreading, and proliferation of human cells (endothelial, epithelial, fibroblast, glial, and osteoblast) by visualizing cells using calcein-acetoxy methyl ester as a vital stain. The results indicated that cells attached, spread, and proliferated on bothn types of hydrogels. In addition, the scaffolds developed can be used for the long-term culturing of human cells.
AB - We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330,μm) to the bottom (20-30 μm). Type II hydrogels contained spherical pores with a diameter of 135,μm. Both types of scaffolds were evaluated by confocal laser scanning microscopy in terms of adhesion, spreading, and proliferation of human cells (endothelial, epithelial, fibroblast, glial, and osteoblast) by visualizing cells using calcein-acetoxy methyl ester as a vital stain. The results indicated that cells attached, spread, and proliferated on bothn types of hydrogels. In addition, the scaffolds developed can be used for the long-term culturing of human cells.
UR - http://www.scopus.com/inward/record.url?scp=33847302529&partnerID=8YFLogxK
U2 - 10.1021/bm0606869
DO - 10.1021/bm0606869
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C2 - 17291056
AN - SCOPUS:33847302529
SN - 1525-7797
VL - 8
SP - 338
EP - 344
JO - Biomacromolecules
JF - Biomacromolecules
IS - 2
ER -