Polymorphism of DNA/Multi-cationic lipid complexes driven by temperature and salts

Eric Raspaud; Bruno Pitard; Dominique Durand; Olivier Aguerre-Chariol; Juan Pelta; Gerardo Byk; Daniel Scherman; Françoise Livolant

doi:10.1021/jp004214e

Polymorphism of DNA/Multi-cationic lipid complexes driven by temperature and salts

Eric Raspaud, Bruno Pitard, Dominique Durand, Olivier Aguerre-Chariol, Juan Pelta, Gerardo Byk, Daniel Scherman, Françoise Livolant

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: A classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

Original language	English
Pages (from-to)	5291-5297
Number of pages	7
Journal	Journal of Physical Chemistry B
Volume	105
Issue number	22
DOIs	https://doi.org/10.1021/jp004214e
State	Published - 7 Jun 2001

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abstract = "The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: A classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.",

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AU - Raspaud, Eric

AU - Pitard, Bruno

AU - Durand, Dominique

AU - Aguerre-Chariol, Olivier

AU - Pelta, Juan

AU - Byk, Gerardo

AU - Scherman, Daniel

AU - Livolant, Françoise

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N2 - The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: A classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

AB - The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: A classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

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Polymorphism of DNA/Multi-cationic lipid complexes driven by temperature and salts

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