Polymorphism of DNA/multi-cationic lipid complexes driven by temperature and salts

Eric Raspaud; Bruno Pitard; Dominique Durand; Olivier Aguerre-Chariol; Juan Pelta; G. Byk; Daniel Scherman; Francoise Livolant

Polymorphism of DNA/multi-cationic lipid complexes driven by temperature and salts

Eric Raspaud, Bruno Pitard, Dominique Durand, Olivier Aguerre-Chariol, Juan Pelta, G. Byk, Daniel Scherman, Francoise Livolant

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: a classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

Original language	American English
Pages (from-to)	5291-5297
Journal	The Journal of Physical Chemistry B
Volume	105
Issue number	22
State	Published - 2001

Cite this

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title = "Polymorphism of DNA/multi-cationic lipid complexes driven by temperature and salts",

abstract = "The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: a classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.",

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T1 - Polymorphism of DNA/multi-cationic lipid complexes driven by temperature and salts

AU - Raspaud, Eric

AU - Pitard, Bruno

AU - Durand, Dominique

AU - Aguerre-Chariol, Olivier

AU - Pelta, Juan

AU - Byk, G.

AU - Scherman, Daniel

AU - Livolant, Francoise

PY - 2001

Y1 - 2001

N2 - The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: a classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

AB - The structural polymorphism of DNA/lipopolyamine (multi-cationic lipid) complexes has been studied by X-ray diffraction and cryo-electron microscopy. Monovalent salt and temperature effects have been analyzed. Depending on the treatment applied to the lipidic solution prior to DNA addition, two types of structure can be obtained for identical final conditions: a classical lamellar structure and a more complex structure. The latter was shown to appear when the lipidic solution prepared in high salt condition is vortexed and quenched below the hydrocarbon chain melting temperature, before addition to the DNA solution. It corresponds to some small facetted vesicles. Similar behavior is observed for the poly(styrene-sulfonate)/lipid system.

UR - https://scholar.google.co.il/scholar?q=Polymorphism+of+DNA%2FMulti-cationic+Lipid+Complexes+Driven+by+Temperature+and+Salts&btnG=&hl=en&as_sdt=0%2C5

M3 - Article

VL - 105

SP - 5291

EP - 5297

JO - The Journal of Physical Chemistry B

JF - The Journal of Physical Chemistry B

IS - 22

ER -

Polymorphism of DNA/multi-cationic lipid complexes driven by temperature and salts

Abstract

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