Polymer-supported nucleic acid fragments. Tools for biotechnology and biomedical research

H. Seliger; R. Bader; M. Hinz; B. Rotte; F. Eisenbeiss; S. Gura; B. Nitzan; S. Margel

doi:10.1016/S1381-5148(99)00064-4

Polymer-supported nucleic acid fragments. Tools for biotechnology and biomedical research

H. Seliger
, R. Bader
, M. Hinz
, B. Rotte
, F. Eisenbeiss
, S. Gura
, B. Nitzan
, S. Margel

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A survey is given on recent work in the authors' laboratories dealing with the preparation and application of functional polymers, which are to be used (a) as supports for nucleic acid synthesis and (b) as carriers mediating the interaction of the immobilized nucleic acid chains with dissolved macromolecules. Two systems are spotlighted, which require loading capacities (1) as high as possible, or (2) as low as possible. These are (1) libraries of oligonucleotides bound either to particles or to a polypropylene tape. Ad (2) the possibility of loading a carrier with a single DNA molecule for exonucleolytic sequence analysis is discussed. The requirements for appropriate functional polymers for both applications are in the focus of this article.

Original language	English
Pages (from-to)	325-339
Number of pages	15
Journal	Reactive and Functional Polymers
Volume	43
Issue number	3
DOIs	https://doi.org/10.1016/S1381-5148(99)00064-4
State	Published - 15 Mar 2000

Bibliographical note

Funding Information:
Partial support of this work was obtained from the Deutsche Forschungsgemeinschaft (Graduiertenkolleg Biomolekulare Medizin), from the German Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (Grant no. 0310794-659), from the EU Copernicus Program (Grant no. CIPACT-93-0155 (D6 12 HSMU)) and from the German–Israeli Research Foundation. The studies in Israel were partially supported by Minerva (Otto Meyerhoff Center for the Study of Drug–Receptor Interactions).

Funding

Partial support of this work was obtained from the Deutsche Forschungsgemeinschaft (Graduiertenkolleg Biomolekulare Medizin), from the German Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (Grant no. 0310794-659), from the EU Copernicus Program (Grant no. CIPACT-93-0155 (D6 12 HSMU)) and from the German–Israeli Research Foundation. The studies in Israel were partially supported by Minerva (Otto Meyerhoff Center for the Study of Drug–Receptor Interactions).

Funders	Funder number
EU Copernicus Program	CIPACT-93-0155
German–Israeli Research Foundation
Deutsche Forschungsgemeinschaft
Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie	0310794-659

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10.1016/S1381-5148(99)00064-4

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abstract = "A survey is given on recent work in the authors' laboratories dealing with the preparation and application of functional polymers, which are to be used (a) as supports for nucleic acid synthesis and (b) as carriers mediating the interaction of the immobilized nucleic acid chains with dissolved macromolecules. Two systems are spotlighted, which require loading capacities (1) as high as possible, or (2) as low as possible. These are (1) libraries of oligonucleotides bound either to particles or to a polypropylene tape. Ad (2) the possibility of loading a carrier with a single DNA molecule for exonucleolytic sequence analysis is discussed. The requirements for appropriate functional polymers for both applications are in the focus of this article.",

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AU - Gura, S.

AU - Nitzan, B.

AU - Margel, S.

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Polymer-supported nucleic acid fragments. Tools for biotechnology and biomedical research

Abstract

Bibliographical note

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