TY - JOUR
T1 - Polymer live-cell array for real-time kinetic imaging of immune cells
AU - Zurgil, Naomi
AU - Afrimzon, Elena
AU - Deutsch, Assaf
AU - Namer, Yaniv
AU - Shafran, Yana
AU - Sobolev, Maria
AU - Tauber, Yishay
AU - Ravid-Hermesh, Orit
AU - Deutsch, Mordechai
N1 - Funding Information:
This research was supported by the Horowitz Foundation .
PY - 2010/6
Y1 - 2010/6
N2 - Direct quantitative experimental investigations of the function of lymphocytes and other immune cells are challenging due to the cell mobility and the complexity of intercellular communications. In order to facilitate such investigations, an in vitro system is required that is noninvasive and provides kinetic data on cellular responses to challenges such as drug treatments. The present work reports the development of a disposable, inexpensive polymer-made device, the Polymer Live Cell Array (PLCA), for real-time, kinetic analysis of immune cells. The PLCA proved to be optically and biologically compatible, thus individual immune cells can be observed and treated independently without being tethered. The cells share a common space which facilitates cellular communications via secreted molecules or via direct intercellular interactions. These properties facilitate real-time, non-intrusive, repeated measurements of immune cells under multiple experimental treatments.
AB - Direct quantitative experimental investigations of the function of lymphocytes and other immune cells are challenging due to the cell mobility and the complexity of intercellular communications. In order to facilitate such investigations, an in vitro system is required that is noninvasive and provides kinetic data on cellular responses to challenges such as drug treatments. The present work reports the development of a disposable, inexpensive polymer-made device, the Polymer Live Cell Array (PLCA), for real-time, kinetic analysis of immune cells. The PLCA proved to be optically and biologically compatible, thus individual immune cells can be observed and treated independently without being tethered. The cells share a common space which facilitates cellular communications via secreted molecules or via direct intercellular interactions. These properties facilitate real-time, non-intrusive, repeated measurements of immune cells under multiple experimental treatments.
KW - Biocompatibility
KW - Cell retaining technology
KW - In vitro single-cell analysis
KW - Kinetic individual cell measurement
KW - Lymphocyte imaging
KW - Polymer live cell array
UR - http://www.scopus.com/inward/record.url?scp=77951977059&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2010.02.035
DO - 10.1016/j.biomaterials.2010.02.035
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C2 - 20346503
AN - SCOPUS:77951977059
SN - 0142-9612
VL - 31
SP - 5022
EP - 5029
JO - Biomaterials
JF - Biomaterials
IS - 18
ER -