Polymer live-cell array for real-time kinetic imaging of immune cells

Naomi Zurgil, Elena Afrimzon, Assaf Deutsch, Yaniv Namer, Yana Shafran, Maria Sobolev, Yishay Tauber, Orit Ravid-Hermesh, Mordechai Deutsch

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Direct quantitative experimental investigations of the function of lymphocytes and other immune cells are challenging due to the cell mobility and the complexity of intercellular communications. In order to facilitate such investigations, an in vitro system is required that is noninvasive and provides kinetic data on cellular responses to challenges such as drug treatments. The present work reports the development of a disposable, inexpensive polymer-made device, the Polymer Live Cell Array (PLCA), for real-time, kinetic analysis of immune cells. The PLCA proved to be optically and biologically compatible, thus individual immune cells can be observed and treated independently without being tethered. The cells share a common space which facilitates cellular communications via secreted molecules or via direct intercellular interactions. These properties facilitate real-time, non-intrusive, repeated measurements of immune cells under multiple experimental treatments.

Original languageEnglish
Pages (from-to)5022-5029
Number of pages8
JournalBiomaterials
Volume31
Issue number18
DOIs
StatePublished - Jun 2010

Bibliographical note

Funding Information:
This research was supported by the Horowitz Foundation .

Keywords

  • Biocompatibility
  • Cell retaining technology
  • In vitro single-cell analysis
  • Kinetic individual cell measurement
  • Lymphocyte imaging
  • Polymer live cell array

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