Abstract
Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hydrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.
| Original language | English |
|---|---|
| Pages (from-to) | 239-250 |
| Number of pages | 12 |
| Journal | Journal of Immunological Methods |
| Volume | 24 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Dec 1978 |
| Externally published | Yes |
Bibliographical note
Funding Information:We wish to thank Z. Timor for assistance in labeling of human lymphocytes. This investigation was supported by Grant No 1R01-CA20668-01 awarded by National Cancer Institute, DHEW.
Funding
We wish to thank Z. Timor for assistance in labeling of human lymphocytes. This investigation was supported by Grant No 1R01-CA20668-01 awarded by National Cancer Institute, DHEW.
| Funders |
|---|
| National Cancer Institute |
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