Abstract
Procedures were developed for the synthesis of a new immunoreagent in form of polyglutaraldehyde (PGL) microspheres in sizes ranging from about 50 nm to 1.5 μm. Addition of fluorochromes during synthesis yielded microspheres of high fluorescence intensity. By carrying out the polymerization of glutaraldehyde in presence of iron oxide, magnetic PGL microspheres were produced. Antibody conjugates obtained by interaction of PGL microspheres with immunoglobulins were used to label human red blood cells (RBC) and lymphocytes. A simple method for the separation of magnetically labeled human RBC from unlabeled cells was demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 341-353 |
| Number of pages | 13 |
| Journal | Journal of Immunological Methods |
| Volume | 28 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - 24 Jul 1979 |
| Externally published | Yes |
Bibliographical note
Funding Information:This report representos ne phaseo f researchca rriedout at the Jet Propulsion Laboratory, California Institute of Technology under Contract No. NAST-100 s p o n s o r e db y the National A e r o n a u t i c sa n d Space Administration and by Grant No. 1RO1-CA20668-01 awarded by the National Cancer InstituteD, HEW.