Polyglutaraldehyde: A new reagent for coupling proteins to microspheres and for labeling cell-surface receptors

A. Rembaum, S. Margel, J. Levy

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hydrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.

Original languageEnglish
Pages (from-to)239-250
Number of pages12
JournalJournal of Immunological Methods
Volume24
Issue number3-4
DOIs
StatePublished - Dec 1978
Externally publishedYes

Bibliographical note

Funding Information:
We wish to thank Z. Timor for assistance in labeling of human lymphocytes. This investigation was supported by Grant No 1R01-CA20668-01 awarded by National Cancer Institute, DHEW.

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