Polyacrolein microspheres as a new solid phase for radioimmunoassay

M. Pines, S. Margel

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Polyacrolein (PA) microspheres contain reactive aldehyde groups through which ligands containing primary amino groups such as proteins and drugs can be covalently bound in a single step at physiological pH. Antibodies against cyclic-AMP, digoxin and rabbit serum were thus coupled to PA microspheres. The immuno-microspheres were kept in suspension or freeze-dried, with insignificant decrease in their binding capacity. The conjugates were used in the respective radioimmunoassay (RIA) systems to facilitate the separation of the free and the antibody-bound 125I ligands, in comparison with precipitation of Protein A of Staphylococcus aureus. Cyclic-AMP was assayed using PA microspheres coupled either with the primary antibody or with anti-rabbit serum as a secondary antibody, in a buffer system, in chick plasma, in urine and in media in which avian dispersed kidney cells had been stimulated by various agents. The results obtained using the immuno-microspheres and the bacterial separation methods were indistinguishable. Other 125I-ligands, such as digoxin in buffer system or thyroxine and triiodothyronine in chick plasma, were assayed in the picogram range. Owing to the solubility of non crosslinked microspheres conjugates in toluene-based scintillation fluids, both the free and the bound fractions could be counted when using 3H-ligands. Corticosterone was assayed using this technique.

Original languageEnglish
Pages (from-to)97-111
Number of pages15
JournalJournal of Immunoassay
Volume7
Issue number1-2
DOIs
StatePublished - 1 Mar 1986
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grant no. 3560922-83 from Galil Advanced Technologies, Israel. Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel (no. 1136E, 1984 series).

Funding

This work was supported by grant no. 3560922-83 from Galil Advanced Technologies, Israel. Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel (no. 1136E, 1984 series).

FundersFunder number
Galil Advanced Technologies, Israel

    Keywords

    • Polyacrolein Beads
    • Polyacrolein Microspheres
    • Radioimmunoassay
    • Solid Phase

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