Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Asaf Grupi; Zehavit Shapira; Shimon Yudovich; Nurit Degani-Katzav; Shimon Weiss

doi:10.1016/j.bpr.2021.100007

Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Asaf Grupi, Zehavit Shapira, Shimon Yudovich, Nurit Degani-Katzav, Shimon Weiss

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA_A antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

Original language	English
Article number	100007
Journal	Biophysical Reports
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.bpr.2021.100007
State	Published - 8 Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s)

Funding

We thank Professor Dan Oron and Dr. Miri Kazes help with quantum yield measurements and for helpful discussions and Eugene Brozgol and Professor Yuval Garini for help with lifetime measurements. This work has received funding from the European Research Council under the European Union's Horizon 2020 research and innovation program under grant agreement number 669941, by the Human Frontier Science Program research grant RGP0061/2015, by the BER program of the Department of Energy Office of Science grant DE-SC0020338, by the STROBE National Science Foundation Science & Technology Center grant number DMR-1548924, by the Israel Science Foundation grant 813/19, and by the Bar-Ilan Research & Development Co., the Israel Innovation Authority, grant number 63392. This work has received funding from the European Research Council under the European Union’s Horizon 2020 research and innovation program under grant agreement number 669941 , by the Human Frontier Science Program research grant RGP0061/2015 , by the BER program of the Department of Energy Office of Science grant DE-SC0020338 , by the STROBE National Science Foundation Science & Technology Center grant number DMR-1548924 , by the Israel Science Foundation grant 813/19 , and by the Bar-Ilan Research & Development Co. , the Israel Innovation Authority , grant number 63392 .

Funders	Funder number
Bar-Ilan Research & Development Co.
Israel Innovation Authority	63392
STROBE National Science Foundation Science & Technology Center	DMR-1548924
Office of Science	DE-SC0020338
Horizon 2020 Framework Programme
European Commission
Human Frontier Science Program	RGP0061/2015
Israel Science Foundation	813/19
Horizon 2020	669941

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Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Abstract

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Funding

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