Plant regeneration via somatic embryogenesis from root explants of Daucus carota L. subsp. halophilus

A system for induction of callus and plant regeneration via somatic embryogenesis from root explants of Daucus carota L. sub sp. halophilus was evaluated. The influence of plant growth regulators (PGRs) including 2,4- dichlorophenoxyacetic acid (0.25-2.50 mg/L^-1 2,4-D) on callus induction from root explants of in vitro plantlets was studied. 2,4-dichlorophenoxyacetic acid (2,4-D), and Kinetin were used for somatic embryogenesis. The results showed that the highest (100%) callus induction on Murashige and Skoog (MS) medium supplemented with 2,4-D (0.75 mg/L^-1) and highest rate (100%) of callus multiplication in MS + 2,4-D (1.5 mg/L^-1) + NAA (0.50 mg/L^-1)was observed. The highest induction frequency of embryogenic callus emerged on Murashige and Skoog (MS) medium supplemented with MS + 2,4-D (1.5 mg/L^-1) + Kn (0.25 mg/L^-1). Mean of 4 somatic embryos per embryogenic callus were obtained and approximately 90 % of them developed into plantlets. The plant regeneration system established in this study will facilitate mass propagation and may be applied to culture of the roots of wild Daucus carota.