TY - JOUR
T1 - Physical and biophysical characteristics of nanoscale tungsten oxide particles and their interaction with human genomic DNA
AU - Kumar, Vijay Bhooshan
AU - Sawian, Clara Ermine
AU - Mohanta, Dambarudhar
AU - Baruah, Shashi
AU - Islam, Nashreen S.
PY - 2011/6
Y1 - 2011/6
N2 - Nanoscale tungsten oxide (WO 3) particles were synthesized via a user-friendly solvothermal cum reduction route using sodium tungstanate (Na 2WO 4) and cetyl trimethyl ammonium bromide (C 19H 42NBr) as reactants. The X-ray diffraction and transmission electron microscopy studies have revealed monoclinic phase of WO 3 with an average crystallite size of ̃40 nm and competitive crystallographic orientation along (002), (020), (200) planes. After extracting human genomic DNA from human blood by a standard protocol (Qiagen-Kit method®), they were conjugated with nanoscale WO 3 particles in varying molar concentrations. The biophysical interaction of DNA bound nanoparticles were characterized by Fourier transform infra-red spectroscopy, photoluminescence spectroscopy, agarose gel-electrophoresis and polymerase chain reaction. Understanding physical and biophysical aspects of unconjugated and DNA conjugated WO 3 would provide scope for biosensing applications.
AB - Nanoscale tungsten oxide (WO 3) particles were synthesized via a user-friendly solvothermal cum reduction route using sodium tungstanate (Na 2WO 4) and cetyl trimethyl ammonium bromide (C 19H 42NBr) as reactants. The X-ray diffraction and transmission electron microscopy studies have revealed monoclinic phase of WO 3 with an average crystallite size of ̃40 nm and competitive crystallographic orientation along (002), (020), (200) planes. After extracting human genomic DNA from human blood by a standard protocol (Qiagen-Kit method®), they were conjugated with nanoscale WO 3 particles in varying molar concentrations. The biophysical interaction of DNA bound nanoparticles were characterized by Fourier transform infra-red spectroscopy, photoluminescence spectroscopy, agarose gel-electrophoresis and polymerase chain reaction. Understanding physical and biophysical aspects of unconjugated and DNA conjugated WO 3 would provide scope for biosensing applications.
KW - Bioconjugation
KW - DNA
KW - FTIR
KW - Nanoparticles
KW - Photoluminescence
KW - Tungsten O ide
UR - http://www.scopus.com/inward/record.url?scp=80051747778&partnerID=8YFLogxK
U2 - 10.1166/jnn.2011.4122
DO - 10.1166/jnn.2011.4122
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 21770090
AN - SCOPUS:80051747778
SN - 1533-4880
VL - 11
SP - 4659
EP - 4666
JO - Journal of Nanoscience and Nanotechnology
JF - Journal of Nanoscience and Nanotechnology
IS - 6
ER -