Photon-counting H33D detector for biological fluorescence imaging

X. Michalet; O. H.W. Siegmund; J. V. Vallerga; P. Jelinsky; J. E. Millaud; S. Weiss

doi:10.1016/j.nima.2006.05.155

Photon-counting H33D detector for biological fluorescence imaging

X. Michalet
, O. H.W. Siegmund
, J. V. Vallerga
, P. Jelinsky
, J. E. Millaud
, S. Weiss

University of California at Los Angeles
University of California at Berkeley
Lawrence Livermore National Laboratory

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

Original language	English
Pages (from-to)	133-136
Number of pages	4
Journal	Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume	567
Issue number	1 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.nima.2006.05.155
State	Published - 1 Nov 2006
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by NIH Grant 5 R21 RR017474 and in part by NIH Grant 5 R01 EB000312. We would like to thank F.F. Pinaud, J.M. Tsay and Drs. J.J. Li and G. Iyer for providing fluorescent samples and cells. The contribution of J. Malloy for help in the photocathode deposition, C. Scholz for electronics development and Drs A. Tremsin and B. Welsh for helpful discussions is also gratefully acknowledged.

Funding

This work was supported by NIH Grant 5 R21 RR017474 and in part by NIH Grant 5 R01 EB000312. We would like to thank F.F. Pinaud, J.M. Tsay and Drs. J.J. Li and G. Iyer for providing fluorescent samples and cells. The contribution of J. Malloy for help in the photocathode deposition, C. Scholz for electronics development and Drs A. Tremsin and B. Welsh for helpful discussions is also gratefully acknowledged.

Funders	Funder number
National Institutes of Health	5 R01 EB000312, 5 R21 RR017474

Keywords

Fluorescence
Lifetime
Photocathode
Photon counting
Position sensitive anode
Quantum dot

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10.1016/j.nima.2006.05.155

Cite this

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abstract = "We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.",

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note = "Funding Information: This work was supported by NIH Grant 5 R21 RR017474 and in part by NIH Grant 5 R01 EB000312. We would like to thank F.F. Pinaud, J.M. Tsay and Drs. J.J. Li and G. Iyer for providing fluorescent samples and cells. The contribution of J. Malloy for help in the photocathode deposition, C. Scholz for electronics development and Drs A. Tremsin and B. Welsh for helpful discussions is also gratefully acknowledged.",

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Photon-counting H33D detector for biological fluorescence imaging. / Michalet, X.; Siegmund, O. H.W.; Vallerga, J. V. et al.
In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 567, No. 1 SPEC. ISS., 01.11.2006, p. 133-136.

Research output: Contribution to journal › Article › peer-review

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T1 - Photon-counting H33D detector for biological fluorescence imaging

AU - Michalet, X.

AU - Siegmund, O. H.W.

AU - Vallerga, J. V.

AU - Jelinsky, P.

AU - Millaud, J. E.

AU - Weiss, S.

N1 - Funding Information: This work was supported by NIH Grant 5 R21 RR017474 and in part by NIH Grant 5 R01 EB000312. We would like to thank F.F. Pinaud, J.M. Tsay and Drs. J.J. Li and G. Iyer for providing fluorescent samples and cells. The contribution of J. Malloy for help in the photocathode deposition, C. Scholz for electronics development and Drs A. Tremsin and B. Welsh for helpful discussions is also gratefully acknowledged.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

AB - We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

KW - Fluorescence

KW - Lifetime

KW - Photocathode

KW - Photon counting

KW - Position sensitive anode

KW - Quantum dot

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JF - Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment

IS - 1 SPEC. ISS.

ER -

Photon-counting H33D detector for biological fluorescence imaging

Abstract

Bibliographical note

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Keywords

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