We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.
|Number of pages
|Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
|1 SPEC. ISS.
|Published - 1 Nov 2006
Bibliographical noteFunding Information:
This work was supported by NIH Grant 5 R21 RR017474 and in part by NIH Grant 5 R01 EB000312. We would like to thank F.F. Pinaud, J.M. Tsay and Drs. J.J. Li and G. Iyer for providing fluorescent samples and cells. The contribution of J. Malloy for help in the photocathode deposition, C. Scholz for electronics development and Drs A. Tremsin and B. Welsh for helpful discussions is also gratefully acknowledged.
- Photon counting
- Position sensitive anode
- Quantum dot