Photoconversion of DAPI following UV or violet excitation can cause DAPI to fluoresce with blue or cyan excitation

M. Piterburg, H. Panet, A. Weiss

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

4'-6-Diamidino-2-phenylindole is a fluorescent dye commonly used to visualize deoxyribonucleic acid or cell nuclei in fixed cell preparations, and is often used together with fluorescein or green fluorescent protein, which can be excited without exciting 4'-6-Diamidino-2-phenylindole. It is assumed that when using typical fluorescein or green fluorescent protein filter cubes, 4'-6-Diamidino-2-phenylindole will not be observed. In this paper, we show that following observation of 4'-6-Diamidino-2-phenylindole using UV or violet excitation, it may become sensitive to the blue/cyan excitation used in fluorescein/green fluorescent protein filter cubes. This has serious implications for the use of 4'-6-Diamidino-2-phenylindole together with widely used green fluorophores in double labelling experiments.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Microscopy
Volume246
Issue number1
DOIs
StatePublished - Apr 2012

Keywords

  • DAPI
  • Fluorescence
  • Photo conversion

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