TY - JOUR
T1 - Photoconversion of DAPI following UV or violet excitation can cause DAPI to fluoresce with blue or cyan excitation
AU - Piterburg, M.
AU - Panet, H.
AU - Weiss, A.
PY - 2012/4
Y1 - 2012/4
N2 - 4'-6-Diamidino-2-phenylindole is a fluorescent dye commonly used to visualize deoxyribonucleic acid or cell nuclei in fixed cell preparations, and is often used together with fluorescein or green fluorescent protein, which can be excited without exciting 4'-6-Diamidino-2-phenylindole. It is assumed that when using typical fluorescein or green fluorescent protein filter cubes, 4'-6-Diamidino-2-phenylindole will not be observed. In this paper, we show that following observation of 4'-6-Diamidino-2-phenylindole using UV or violet excitation, it may become sensitive to the blue/cyan excitation used in fluorescein/green fluorescent protein filter cubes. This has serious implications for the use of 4'-6-Diamidino-2-phenylindole together with widely used green fluorophores in double labelling experiments.
AB - 4'-6-Diamidino-2-phenylindole is a fluorescent dye commonly used to visualize deoxyribonucleic acid or cell nuclei in fixed cell preparations, and is often used together with fluorescein or green fluorescent protein, which can be excited without exciting 4'-6-Diamidino-2-phenylindole. It is assumed that when using typical fluorescein or green fluorescent protein filter cubes, 4'-6-Diamidino-2-phenylindole will not be observed. In this paper, we show that following observation of 4'-6-Diamidino-2-phenylindole using UV or violet excitation, it may become sensitive to the blue/cyan excitation used in fluorescein/green fluorescent protein filter cubes. This has serious implications for the use of 4'-6-Diamidino-2-phenylindole together with widely used green fluorophores in double labelling experiments.
KW - DAPI
KW - Fluorescence
KW - Photo conversion
UR - http://www.scopus.com/inward/record.url?scp=84857994566&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2818.2011.03591.x
DO - 10.1111/j.1365-2818.2011.03591.x
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C2 - 22288651
AN - SCOPUS:84857994566
SN - 0022-2720
VL - 246
SP - 89
EP - 95
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 1
ER -