TY - JOUR
T1 - Photoaffinity labeling on magnetic microspheres (PALMm) methodology for topographic mapping
T2 - Preparation of PALMm reagents and demonstration of biochemical relevance
AU - Halbfinger, Efrat
AU - Gorochesky, Karine
AU - Lévesque, Sébastien A.
AU - Beaudoin, Adrien R.
AU - Sheihet, Larisa
AU - Margel, Shlomo
AU - Fischer, Bilha
PY - 2003/8/21
Y1 - 2003/8/21
N2 - Photoaffinity labeling (PAL) is a technique widely used for identifying the binding-site within proteins. Although the classic method is both versatile and powerful, it suffers significant disadvantages, such as the need to radiolabel the PAL ligand, and the need to conduct highly complicated separations of both the labeled protein and the labeled peptides derived from it. Here, we propose a novel and universal methodology - Photo-Affinity Labeling on Magnetic microspheres (PALMm) designed to simplify and shorten the PAL protocol. In this context, we describe the preparation of PALMm reagents and the evaluation of their biochemical relevance regarding two ATP-binding enzymes: hexokinase and apyrase.
AB - Photoaffinity labeling (PAL) is a technique widely used for identifying the binding-site within proteins. Although the classic method is both versatile and powerful, it suffers significant disadvantages, such as the need to radiolabel the PAL ligand, and the need to conduct highly complicated separations of both the labeled protein and the labeled peptides derived from it. Here, we propose a novel and universal methodology - Photo-Affinity Labeling on Magnetic microspheres (PALMm) designed to simplify and shorten the PAL protocol. In this context, we describe the preparation of PALMm reagents and the evaluation of their biochemical relevance regarding two ATP-binding enzymes: hexokinase and apyrase.
UR - http://www.scopus.com/inward/record.url?scp=0042319308&partnerID=8YFLogxK
U2 - 10.1039/b303425a
DO - 10.1039/b303425a
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SN - 1477-0520
VL - 1
SP - 2821
EP - 2832
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 16
ER -