Photoacoustic calorimetry of concentrated fluorescent solutions

David Cahen, Haim Garty, Ralph S. Becker

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Photoacoustic (PA) detection is used to determine fluorescence quantum yields of concentrated disodium fluorescein (Na2Fl) and cresyl violet perchlorate (CVP) solutions. Use of heat dissipation spectra, which are obtained from the ratio of the corrected photoacoustic and transmission absorption spectra, is found to give the most reliable results. The presence of several species with different fluorescence characteristics, apparent from the PA data, limits the wavelength range over which the dissipation spectrum can be used to obtain quantitative information. The occurrence of PA saturation leads to erroneous results if data for only one wavelength are used; however, its effect cancels when extrapolation of heat dissipation spectra is employed. Characteristic features of PA detection of fluorescence that are noted include the fact that total fluorescence is sensed and that the technique is essentially a thin film reflectance one. Such features, which can complicate direct comparison of PA data with conventional ones, may be used advantageously in specific problems.

Original languageEnglish
Pages (from-to)3384-3389
Number of pages6
JournalJournal of Physical Chemistry
Volume84
Issue number25
DOIs
StatePublished - 1980
Externally publishedYes

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