Abstract
Previous investigation demonstrated the potential of β-phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with β-phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM β-phenylpyruvate in soleus muscle (P < 0.01) and C2C12 cells (P < 0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in Vmax compared with controls (P < 0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to β -phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of β-phenylpyruvate.
Original language | English |
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Pages (from-to) | 957-963 |
Number of pages | 7 |
Journal | Journal of Cellular Biochemistry |
Volume | 90 |
Issue number | 5 |
DOIs | |
State | Published - 1 Dec 2003 |
Externally published | Yes |
Keywords
- Cultured cells
- Glucose
- Glucose transporters
- Phenylketonurias
- Phenylpyruvic acid
- Skeletal muscle