Pharmacological Insights: Mitochondrial ROS Generation by FNC (Azvudine) in Dalton’s Lymphoma Cells Revealed by Super Resolution Imaging

Naveen Kumar, Vikram Delu, Ilya Ulasov, Sanjay Kumar, Rishi Kant Singh, Sandeep Kumar, Alok Shukla, Anand Kumar Patel, Lokesh Yadav, Ruchi Tiwari, Kumari Rachana, Shivashish Priyadarshi Mohanta, Varsha Singh, Anuradha Yadav, Kaushalendra Kaushalendra, Arbind Acharya

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Nucleoside analogs are a common form of chemotherapy that disrupts DNA replication and repair, leading to cell cycle arrest and apoptosis. Reactive oxygen species (ROS) production is a significant mechanism through which these drugs exert their anticancer effects. This study investigated a new nucleoside analog called FNC or Azvudine, and its impact on ROS production and cell viability in Dalton’s lymphoma (DL) cells. The study found that FNC treatment resulted in a time- and dose-dependent increase in ROS levels in DL cells. After 15 and 30 min of treatment with 2 and 1 mg/ml of FNC, mitochondrial ROS production was observed in DL cells. Furthermore, prolonged exposure to FNC caused structural alterations and DNA damage in DL cells. The results suggest that FNC’s ability to impair DL cell viability may be due to its induction of ROS production and indicate a need for further investigation.

Original languageEnglish
Pages (from-to)873-883
Number of pages11
JournalCell Biochemistry and Biophysics
Volume82
Issue number2
DOIs
StatePublished - Jun 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

  • Azvudine
  • Cell survival
  • DNA damage
  • DNA replication
  • Nucleoside analogs

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