Phage display selection and analysis of Ab‐binding epitopes

D. Enshell-Seijffers, Jonathan M. Gershoni

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The identification and characterization of B cell epitopes by combinatorial phage display peptide analyses is based on the principle that unique peptides can be affinity-purified from an enormous collection of random peptides. Moreover, once selected, the peptide sequence can be elucidated; filamentous bacteriophages have been genetically engineered to incorporate the DNA template corresponding to the peptide displayed on its surface. This unit begins with a discussion of some of the factors that distinguish available libraries. Protocols are then provided for affinity selection of antibody-specific phages, determination of phage titer, confirmation and amplification of positive phages, phage characterization, and construction of custom-tailored phages. The selection protocol in this unit is specific and designed for libraries that are used in the authors' laboratory and are based on the fth1 or fd-tet derived vectors. However, information is included for adapting these protocols to the specific requirements of other phage display libraries.
Original languageAmerican English
Title of host publicationCurrent protocols in immunology
EditorsD. Enshell-Seijffers
StatePublished - 2002


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