Abstract
Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.
| Original language | English |
|---|---|
| Pages (from-to) | 9.8.1-9.8.30 |
| Journal | Current Protocols in Immunology |
| Issue number | SUPPL. 86 |
| DOIs | |
| State | Published - Aug 2009 |
| Externally published | Yes |
Keywords
- Epitope mapping
- Mapitope
- Mimotope
- Pepitope
- Peptide mimetics
- Phage display
- Random peptide libraries
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