TY - JOUR
T1 - Phage display selection, analysis, and prediction of B Cell epitopes
AU - Freund, Natalia Tarnovitski
AU - Enshell-Seijffers, David
AU - Gershoni, Jonathan M.
PY - 2009/8
Y1 - 2009/8
N2 - Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.
AB - Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.
KW - Epitope mapping
KW - Mapitope
KW - Mimotope
KW - Pepitope
KW - Peptide mimetics
KW - Phage display
KW - Random peptide libraries
UR - http://www.scopus.com/inward/record.url?scp=68949164705&partnerID=8YFLogxK
U2 - 10.1002/0471142735.im0908s86
DO - 10.1002/0471142735.im0908s86
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C2 - 19653209
AN - SCOPUS:68949164705
SN - 1934-3671
SP - 9.8.1-9.8.30
JO - Current Protocols in Immunology
JF - Current Protocols in Immunology
IS - SUPPL. 86
ER -