Phage display selection, analysis, and prediction of B cell epitopes

Natalia Tarnovitski Freund; David Enshell‐Seijffers; Jonathan M. Gershoni

Phage display selection, analysis, and prediction of B cell epitopes

Natalia Tarnovitski Freund
, David Enshell‐Seijffers
, Jonathan M. Gershoni

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30.

Original language	American English
Title of host publication	Current Protocols in Immunology
Editors	D. Enshell-Seijffers
Publisher	John Wiley & Sons, Inc.
Pages	8-9
State	Published - 2009

Cite this

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AB - Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30.

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ER -

Phage display selection, analysis, and prediction of B cell epitopes

Abstract

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