Phage display selection, analysis, and prediction of B cell epitopes

Natalia Tarnovitski Freund, David Enshell‐Seijffers, Jonathan M. Gershoni

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30.
Original languageAmerican English
Title of host publicationCurrent Protocols in Immunology
EditorsD. Enshell-Seijffers
PublisherJohn Wiley & Sons, Inc.
StatePublished - 2009


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