TY - CHAP
T1 - Phage display selection, analysis, and prediction of B cell epitopes
AU - Freund, Natalia Tarnovitski
AU - Enshell‐Seijffers, David
AU - Gershoni, Jonathan M.
PY - 2009
Y1 - 2009
N2 - Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30.
AB - Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30.
UR - https://scholar.google.co.il/scholar?q=Phage-display+selection%2C+analysis%2C+and+prediction+of+B-cell+epitopes.&btnG=&hl=en&as_sdt=0%2C5
M3 - Chapter
SP - 8
EP - 9
BT - Current Protocols in Immunology
A2 - Enshell-Seijffers, D.
PB - John Wiley & Sons, Inc.
ER -