Peptide-coated semiconductor nanocrv stals for biomedical applications

X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Iyer, S. Weiss

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


We have developed a new functionalization approach for semiconductor nanocrystals based on a single-step exchange of surface ligands with custom-designed peptides. This peptide-coating technique yield small, monodisperse and very stable water-soluble NCs that remain bright and photostable. We have used this approach on several types of core and core-shell NCs in the visible and near-infrared spectrum range and used fluorescence correlation spectroscopy for rapid assessment of the colloidal and photophysical properties of the resulting particles. This peptide coating strategy has several advantages: it yields probes that are immediately biocompatible; it is amenable to improvements of the different properties (solubilization, functionalization, etc) via rational design, parallel synthesis, or molecular evolution; it permits the combination of several functions on individual NCs. These functionalized NCs have been used for diverse biomedical applications. Two are discussed here: single-particle tracking of membrane receptor in live cells and combined fluorescence and PET imaging of targeted delivery in live animals.

Original languageEnglish
Article number09
Pages (from-to)57-68
Number of pages12
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2005
Externally publishedYes
EventGenetically Engineered and Optical Probes for Biomedical Applications III - San Jose, CA, United States
Duration: 25 Jan 200526 Jan 2005


  • FCS
  • Fluorescence
  • Functionalization
  • Live cell
  • Nanocrystal
  • Peptide
  • Photophysics
  • Quantum dots
  • Single-molecule


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