Abstract
The current research was conducted to study genetic polymorphism in partial sequence of Luteinizing hormone receptor (LHR) gene and its possible association with postpartum anoestrus (PPA) in buffaloes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. On the basis of history sixty (n=60) murrah buffaloes were selected and divided into two groups, thirty with PPA (n1=30) and other thirty with normal oestrus cycle (n2=30). Following peripheral blood collection, genomic DNA was isolated. Partial sequence of LHR gene (303 bp) was amplified by PCR and subjected to digestion using HphI restriction enzyme. Resolution of digested product in agarose gel exhibited single pattern of restriction fragments with xx genotype, thereby polymorphism and possible association of partial LHR gene sequence with PPA in buffaloes could not be established.
| Original language | English |
|---|---|
| Pages (from-to) | 247-249 |
| Number of pages | 3 |
| Journal | Veterinary Practitioner |
| Volume | 16 |
| Issue number | 2 |
| State | Published - Dec 2015 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015, Veterinary Practitioner. All rights reserved.
Funding
Authors are highly grateful to the Ministry of Science and Technology, Department of Science and Technology, Technology Bhavan, New Delhi, India for partially funding the research through INSPIRE fellowship. The authors are also thankful to the Directors of Central institute for Research on Buffaloes, Hisar for providing animals and instruments for this study.
| Funders | Funder number |
|---|---|
| Department of Science and Technology, Technology Bhavan | |
| Ministerio de Ciencia y Tecnología |
Keywords
- LHR gene
- Murrah buffaloes
- Polymorphism
- Postpartum anoestrus