Patterning of particles and live cells at single cell resolution

Adar Hacohen, Hadass R. Jessel, Alon Richter-Levin, Orit Shefi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or dicult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from dierent types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 m. Complementary DNA sequences were used to stabilize and hold the patterns together. The system is accurate, flexible, and easy-to-use, and can be automated for larger-scale tasks. Importantly, it maintains the viability of live cells. We provide quantitative measurements of the process and oer a file format for such assemblies.

Original languageEnglish
Article number505
JournalMicromachines
Volume11
Issue number5
DOIs
StatePublished - 1 May 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors.

Keywords

  • Complementary DNA sequences
  • Micromanipulator
  • Micropatterning
  • Single cell resolution

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