Paclitaxel loaded poly (DL lactic acid co castor oil) 60:40 with poloxamer-F68 rod shape cylindrical nanoparticle preparation and in vitro cytotoxicity studies

Nagaraja SreeHarsha, Jagadeesh G. Hiremath, Rajesh Kumar Aitha, Abraham J. Domb, Bandar E. Al-Dhubiab, Chandramouli Ramnarayanan, Abdullah Mossa Alzahrani, Katharigatta N. Venugopala, Sabah H. Akrawi, Mahesh Attimarad, Anroop B. Nair

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

This research presents a thin-film hydration-solvent evaporation method to formulate the paclitaxel loaded poly (DL lactic acid co castor oil) 4:6 with poloxamer-F68 cylindrical shape nanoparticles. The particles were less than 250 nanometers (nm) in size, with an average width of 60 nm and an average length of 100 nm. The percent yield, encapsulation efficiency (EE), and percent drug loading (DL) were detected. This approach produces drug loading values between 5% and 20% w/w. X-ray powder diffraction (XRD) identified the physicochemical properties of nanoparticles differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR). The investigation shows that the drug is molecularly dispersed in polymers or given in an amorphous or semicrystalline state. Horizontal water bath shaker technology considered the in vitro release of PTX loaded nanoparticles under sink conditions. Poly (DL lactic acid co castor oil) 4:6 nanoparticles exhibited a sustained release analysis. At the end of 30 hours, the percent cumulative drug release from the formulations was between 74.52% and 92.87% for F1 and F4. In vitro cytotoxicity assays indicate that PTX having p (DLLA:CO60:40) nanoparticles have a higher cytotoxic effect on MCF-7/ADR.

Original languageEnglish
Pages (from-to)2613-2622
Number of pages10
JournalPolymers for Advanced Technologies
Volume30
Issue number10
DOIs
StatePublished - 1 Oct 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 John Wiley & Sons, Ltd.

Funding

This study was funded by Deanship of Scientific Research, King Faisal University, Al-Ahsa, Kingdom of Saudi Arabia (Grant Number: 17122011).

FundersFunder number
Al-Ahsa17122011
King Faisal University
Deanship of Scientific Research, King Saud University

    Keywords

    • MCF-7/ADR
    • paclitaxel
    • poloxamer F-68
    • poly (DL lactic acid co castor oil) 4:6

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