Abstract
Pseudomonas aeruginosa lectin PA-II agglutinates human peripheral lymphocytes and stimulates mitogenesis (predominantly in T cells), like the plant lectins PHA and Con A. Murine splenocytes are also agglutinated and stimulated by PA-II as by Con A. Sialidase treatment of the human and murine cells enhances their agglutination and augments the stimulation of human lymphocytes at low PA-II concentrations. The PA-II agglutinating and mitogenic effects are specifically inhibited by L-fucose. The bacterial source and the specificity of PA-II for L-fucose are both rare features among the hitherto described mitogenic lectins. However, since this lectin also binds mannose, a mannose-bearing receptor might be involved in its mitogenicity.
| Original language | English |
|---|---|
| Pages (from-to) | 62-66 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 216 |
| Issue number | 1 |
| DOIs | |
| State | Published - 25 May 1987 |
Bibliographical note
Funding Information:This studyr epresentas part of a thesiss ubmitted by D. Avichezer in partial fulfillment of the requirements for a PhD degree from Bar-Ban University. The authors thank Miss Daniella Lustig for the skillful typing of the manuscript. This study was supported by the Mitzi-Dobrin Cancer ResearchF und, Bar-Ilan University.
Keywords
- (Murine splenocyte, Pseudomonas aeruginosa)
- Lymphocyte
- Mitogenic lectin
- PA-II lectin