Oxidation-reduction states of NADH in vivo: From animals to clinical use

Avraham Mayevsky; Britton Chance

doi:10.1016/j.mito.2007.05.001

Oxidation-reduction states of NADH in vivo: From animals to clinical use

Avraham Mayevsky, Britton Chance

The Mina and Everard Goodman Faculty of Life Sciences

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Mitochondrial dysfunction is part of many pathological states in patients, such as sepsis or stroke. Presently, the monitoring of mitochondrial function in patients is extremely rare, even though NADH redox state is routinely measured in experimental animals. In this article, we describe the scientific backgrounds and practical use of mitochondrial NADH fluorescence measurement that was applied to patients in the past few years. In addition to NADH, we optically measured the microcirculatory blood flow and volume, as well as HbO₂ oxygenation, from the same tissue area. The four detected parameters provide real time data on tissue viability, which is critical for patients monitoring.

Original language	English
Pages (from-to)	330-339
Number of pages	10
Journal	Mitochondrion
Volume	7
Issue number	5
DOIs	https://doi.org/10.1016/j.mito.2007.05.001
State	Published - Sep 2007

Bibliographical note

Funding Information:
This work was supported by the Israel Science Foundation Grant number 358/04 and by the Mina and Everard Goodman Faculty of Life Sciences Bar Ilan University, Israel.

Keywords

In vivo organ monitoring
Mitochondrial function
NADH redox state
Tissue blood flow
Tissue energy metabolism
Tissue viability

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10.1016/j.mito.2007.05.001

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abstract = "Mitochondrial dysfunction is part of many pathological states in patients, such as sepsis or stroke. Presently, the monitoring of mitochondrial function in patients is extremely rare, even though NADH redox state is routinely measured in experimental animals. In this article, we describe the scientific backgrounds and practical use of mitochondrial NADH fluorescence measurement that was applied to patients in the past few years. In addition to NADH, we optically measured the microcirculatory blood flow and volume, as well as HbO2 oxygenation, from the same tissue area. The four detected parameters provide real time data on tissue viability, which is critical for patients monitoring.",

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AB - Mitochondrial dysfunction is part of many pathological states in patients, such as sepsis or stroke. Presently, the monitoring of mitochondrial function in patients is extremely rare, even though NADH redox state is routinely measured in experimental animals. In this article, we describe the scientific backgrounds and practical use of mitochondrial NADH fluorescence measurement that was applied to patients in the past few years. In addition to NADH, we optically measured the microcirculatory blood flow and volume, as well as HbO2 oxygenation, from the same tissue area. The four detected parameters provide real time data on tissue viability, which is critical for patients monitoring.

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Oxidation-reduction states of NADH in vivo: From animals to clinical use

Abstract

Bibliographical note

Keywords

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