Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad

Chloé Mayère, Violaine Regard, Aitana Perea-Gomez, Corey Bunce, Yasmine Neirijnck, Cyril Djari, Natividad Bellido-Carreras, Pauline Sararols, Richard Reeves, Simon Greenaway, Michelle Simon, Pam Siggers, Diana Condrea, Françoise Kühne, Ivana Gantar, Furong Tang, Isabelle Stévant, Laura Batti, Norbert B. Ghyselinck, Dagmar WilhelmAndy Greenfield, Blanche Capel, Marie Christine Chaboissier, Serge Nef

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25 Scopus citations

Abstract

Gonadal sex determination represents a unique model for studying cell fate decisions. However, a complete understanding of the different cell lineages forming the developing testis and ovary remains elusive. Here, we investigated the origin, specification, and subsequent sex-specific differentiation of a previously uncharacterized population of supporting-like cells (SLCs) in the developing mouse gonads. The SLC lineage is closely related to the coelomic epithelium and specified as early as E10.5, making it the first somatic lineage to be specified in the bipotential gonad. SLC progenitors are localized within the genital ridge at the interface with the mesonephros and initially coexpress Wnt4 and Sox9. SLCs become sexually dimorphic around E12.5, progressively acquire a more Sertoli- or pregranulosa-like identity and contribute to the formation of the rete testis and rete ovarii. Last, we found that WNT4 is a crucial regulator of the SLC lineage and is required for normal development of the rete testis.

Original languageEnglish
Article numbereabm0972
JournalScience advances
Volume8
Issue number21
DOIs
StatePublished - 27 May 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2022 The Authors, some rights reserved.

Funding

Swiss National Science Foundation grant 31003A_173070 (S.N.), Swiss National Science Foundation grant 310030_200316 (S.N.), Département de l'Instruction Publique of the State of Geneva (S.N.), Agence Nationale de la Recherche grant ANR-19-CE14-0022-01 SexDiff (M.-C.C.), UK Medical Research Council grant MC_U142684167 (A.G.), National Institutes of Health grant 1R01HD090050 (B.C. and C.B.), and National Institutes of Health grant R37HD039963 (B.C.). N.B.G. and C.D. were supported by grants from Agence Nationale de la Recherche (ANR-16-CE14-0017 and ANR-20-CE14-0022).

FundersFunder number
Département de l'Instruction Publique of the State of Geneva
National Institutes of HealthANR-20-CE14-0022, ANR-16-CE14-0017, 1R01HD090050
National Institute of Child Health and Human DevelopmentR01HD039963
Medical Research CouncilMC_U142684167
Agence Nationale de la RechercheANR-19-CE14-0022-01
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung310030_200316, 31003A_173070

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