Abstract
In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.
Original language | English |
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Pages (from-to) | 5338-5350 |
Number of pages | 13 |
Journal | Biomedical Optics Express |
Volume | 12 |
Issue number | 9 |
DOIs | |
State | Published - 1 Sep 2021 |
Bibliographical note
Publisher Copyright:© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Funding
Israel Science Foundation (1142/15, 2481/19); Ministry of Science, Technology and Space (101790).
Funders | Funder number |
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Ministry of Science, Technology and Space | 101790 |
Israel Science Foundation | 2481/19, 1142/15 |