Optical modulation biosensing system for rapid detection of biological targets at low concentrations

Michael Margulis, Meir Cohen, Shmuel Burg, Shira Avivi-Mintz, Amos Danielli

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In many sensitive assays, target molecules are tagged using fluorescently labeled probes and captured using magnetic beads. Here, we introduce an optical modulation biosensing (OMB) system, which aggregates the beads into a small detection area and separates the signal from the background noise by manipulating the laser beam in and out of the cluster of beads. Using the OMB system to detect human interleukin-8, we demonstrated a limit of detection of 0.02 ng/L and a 4-log dynamic range. Using Zika-positive and healthy individuals' serum samples, we show that the OMB-based Zika IgG serological assay has 96% sensitivity and 100% specificity.

Original languageEnglish
Pages (from-to)5338-5350
Number of pages13
JournalBiomedical Optics Express
Volume12
Issue number9
DOIs
StatePublished - 1 Sep 2021

Bibliographical note

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

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