Optical assessment of intravascular and intracellular parameters related to tissue viability

Avraham Mayevsky, Efrat Sherman, Meir Cohen-Kashi, Nava Dekel, Eliyahu Pewzner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations


Tissue viability represents the balance between O2 supply and demand. In our previous paper (Mayevsky et al; Proc.SPIE 6083 : z1-z10, 2006) the HbO2 was added to the multiparametric tissue spectroscope (Mayevsky et al J.Biomedical Optics 9:1028-1045,2004). This parameter provides relative values of microcirculatory blood oxygenation (MC-HbO2) evaluated by the 2 wavelength reflectometry principle. The advantage of this approach as compared to pulse oximetry is that the measurement is not dependent of the existence of the pulse of the heart. Also in the MC-HbO2 the information is collected from small vessels providing O2 to the mitochondria as compared to the pulse oximeter indicating blood oxygenation by the respiratory and cardiovascular systems. In the present study we compared the level of blood oxygenation measured by the pulse oximeter to that measured by the CritiView in the brain exposed to various systemic and localized perturbations of O2 supply or demand. We exposed gerbils to anoxia, hypoxia, ischemia and terminal anoxia. In addition we measured mitochondrial NADH (surface fluorometry), tissue reflectance, tissue blood flow (laser Doppler flowmetry) from the same site of MC-HbO2 measurement. A clear connection was found between the two blood oxygenation parameters only when systemic perturbations were used (anoxia, hypoxia and terminal anoxia). Under local events (ischemia) the MC-HbO2 was responsive while the systemic oxygenation was unchanged. We concluded that MC-HbO2 has a significant value in interpretation of tissue energy metabolism under pathophysiological conditions.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems V
StatePublished - 2007
EventAdvanced Biomedical and Clinical Diagnostic Systems V - San Jose, CA, United States
Duration: 21 Jan 200727 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvanced Biomedical and Clinical Diagnostic Systems V
Country/TerritoryUnited States
CitySan Jose, CA


  • Microcirculatory oxygenation
  • Mitochondrial function
  • NADH fluorescence
  • Pulse oximetry
  • Tissue viability


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