Open state block by fendiline of L-type Ca++ channels in ventricular myocytes from rat heart

Hermann Nawrath, Gunnar Klein, Johanna Rupp, Jörg W. Wegener, Asher Shainberg

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The effects of fendiline on L-type Ca++ currents [I(Ca(L))] were investigated in rat ventricular cardiomyocytes using the patch-clamp technique both in the whole-cell disrupted-patch and in the cell-attached configuration. For comparison, the effects of verapamil were also investigated. Both compounds depressed the magnitude of whole cell I(Ca(L), verapamil being about 15 times more potent than fendiline. Verapamil did not change the time course of the current, whereas fendiline accelerated its decay when either Ca++ or Ba++ ions were used as charge carriers. In the presence of the Ca++ agonist BayK8644 (10 μM), the potency ratio of fendiline/verapamil was inverted. BayK8644 (10 μM) also reversed the potency ratio of verapamil/fendiline in smooth muscle, with respect to changes in tension induced by K+ (48 mM). In single channel recordings at 0.1 Hz, in the presence of BayK8644 (1 μM) and using Ba++ ions as the charge carrier, fendiline (1 μM) reduced mean open time by 34% and channel availability by 8%; the ensemble average current of Ca++ channels was reduced by 43%. In the same experimental conditions, verapamil (1 μM) was ineffective. These results can be explained by the assumption that fendiline blocks Ca++ channels preferentially in the open state, in contrast to verapamil which blocks preferentially inactivated Ca++ channels.

Original languageEnglish
Pages (from-to)546-552
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Issue number2
StatePublished - May 1998


Dive into the research topics of 'Open state block by fendiline of L-type Ca++ channels in ventricular myocytes from rat heart'. Together they form a unique fingerprint.

Cite this