On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose

Nachman Garber, Ulrike Guempel, Aviva Belz, Nechama Gilboa-Garber, Ronald J. Doyle

    Research output: Contribution to journalArticlepeer-review

    91 Scopus citations

    Abstract

    The d-galactose-binding lectin (PA-I) from the bacterium Pseudomonas aeruginosa, isolated by affinity chromatography on Sepharose, was examined for its relative affinities for simple sugars and their derivatives using equilibrium dialysis and hemagglutination inhibition tests. The lectin, which was found to bind 0.68 mol of d-galactose per subunit of 12.8 kDa, exhibited an association constant (Ka) of 3.4·104 M-1 for d-galactose and higher affinities for hydrophobic and thio derivatives of d-galactose (with highest affinity for the hydrophobic thio derivatives). α-Methyl-galactoside was a stronger inhibitor than the β-methyl derivative and α-lactose was a weak inhibitor but the hydrophobic phenylated derivatives of the β-configuration of d-galactose were more potent inhibitors than the respective α-galactosides.

    Original languageEnglish
    Pages (from-to)331-333
    Number of pages3
    JournalBiochimica et Biophysica Acta - General Subjects
    Volume1116
    Issue number3
    DOIs
    StatePublished - 12 Jun 1992

    Bibliographical note

    Funding Information:
    This work was made possibleb y a sabbaticalle ave given to Professor N. Gather to work at Louisville UniversityK, Y, The researchw ass upportedin part by grantsf romthe March of Dimes (Ohio Valley Chapter) and the U.S.-Israel Bi-Natioual Science Foundation (BSF), JerusalemI,s rael(Grant No. 89.00454).

    Funding

    This work was made possibleb y a sabbaticalle ave given to Professor N. Gather to work at Louisville UniversityK, Y, The researchw ass upportedin part by grantsf romthe March of Dimes (Ohio Valley Chapter) and the U.S.-Israel Bi-Natioual Science Foundation (BSF), JerusalemI,s rael(Grant No. 89.00454).

    FundersFunder number
    Louisville UniversityK
    U.S.-Israel Bi-Natioual Science Foundation
    March of Dimes Foundation
    United States-Israel Binational Science Foundation89.00454

      Keywords

      • (P. aeruginosa)
      • Equilibrium dialysis
      • Hemagglutination
      • Hydrophobic thiosaccharide
      • Lectin
      • d-Galactose

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