On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose

Nachman Garber; Ulrike Guempel; Aviva Belz; Nechama Gilboa-Garber; Ronald J. Doyle

doi:10.1016/0304-4165(92)90048-Y

On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose

Nachman Garber, Ulrike Guempel, Aviva Belz, Nechama Gilboa-Garber, Ronald J. Doyle

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

The d-galactose-binding lectin (PA-I) from the bacterium Pseudomonas aeruginosa, isolated by affinity chromatography on Sepharose, was examined for its relative affinities for simple sugars and their derivatives using equilibrium dialysis and hemagglutination inhibition tests. The lectin, which was found to bind 0.68 mol of d-galactose per subunit of 12.8 kDa, exhibited an association constant (K_a) of 3.4·10⁴ M^-1 for d-galactose and higher affinities for hydrophobic and thio derivatives of d-galactose (with highest affinity for the hydrophobic thio derivatives). α-Methyl-galactoside was a stronger inhibitor than the β-methyl derivative and α-lactose was a weak inhibitor but the hydrophobic phenylated derivatives of the β-configuration of d-galactose were more potent inhibitors than the respective α-galactosides.

Original language	English
Pages (from-to)	331-333
Number of pages	3
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1116
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(92)90048-Y
State	Published - 12 Jun 1992

Bibliographical note

Funding Information:
This work was made possibleb y a sabbaticalle ave given to Professor N. Gather to work at Louisville UniversityK, Y, The researchw ass upportedin part by grantsf romthe March of Dimes (Ohio Valley Chapter) and the U.S.-Israel Bi-Natioual Science Foundation (BSF), JerusalemI,s rael(Grant No. 89.00454).

Funding

This work was made possibleb y a sabbaticalle ave given to Professor N. Gather to work at Louisville UniversityK, Y, The researchw ass upportedin part by grantsf romthe March of Dimes (Ohio Valley Chapter) and the U.S.-Israel Bi-Natioual Science Foundation (BSF), JerusalemI,s rael(Grant No. 89.00454).

Funders	Funder number
Louisville UniversityK
U.S.-Israel Bi-Natioual Science Foundation
March of Dimes Foundation
United States-Israel Binational Science Foundation	89.00454

Keywords

(P. aeruginosa)
Equilibrium dialysis
Hemagglutination
Hydrophobic thiosaccharide
Lectin
d-Galactose

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10.1016/0304-4165(92)90048-Y

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title = "On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose",

abstract = "The d-galactose-binding lectin (PA-I) from the bacterium Pseudomonas aeruginosa, isolated by affinity chromatography on Sepharose, was examined for its relative affinities for simple sugars and their derivatives using equilibrium dialysis and hemagglutination inhibition tests. The lectin, which was found to bind 0.68 mol of d-galactose per subunit of 12.8 kDa, exhibited an association constant (Ka) of 3.4·104 M-1 for d-galactose and higher affinities for hydrophobic and thio derivatives of d-galactose (with highest affinity for the hydrophobic thio derivatives). α-Methyl-galactoside was a stronger inhibitor than the β-methyl derivative and α-lactose was a weak inhibitor but the hydrophobic phenylated derivatives of the β-configuration of d-galactose were more potent inhibitors than the respective α-galactosides.",

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author = "Nachman Garber and Ulrike Guempel and Aviva Belz and Nechama Gilboa-Garber and Doyle, {Ronald J.}",

note = "Funding Information: This work was made possibleb y a sabbaticalle ave given to Professor N. Gather to work at Louisville UniversityK, Y, The researchw ass upportedin part by grantsf romthe March of Dimes (Ohio Valley Chapter) and the U.S.-Israel Bi-Natioual Science Foundation (BSF), JerusalemI,s rael(Grant No. 89.00454).",

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On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose. / Garber, Nachman; Guempel, Ulrike; Belz, Aviva et al.
In: Biochimica et Biophysica Acta - General Subjects, Vol. 1116, No. 3, 12.06.1992, p. 331-333.

Research output: Contribution to journal › Article › peer-review

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AU - Gilboa-Garber, Nechama

AU - Doyle, Ronald J.

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On the specificity of the d-galactose-binding lectin (PA-I) of Pseudomonas aeruginosa and its strong binding to hydrophobic derivatives of d-galactose and thiogalactose

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